1.
Indian J Exp Biol
;
2001 Sep; 39(9): 947-9
Article
in English
| IMSEAR
| ID: sea-62624
ABSTRACT
A protocol was developed for high frequency plant regeneration in H. patulum by shoot-tip culture. H. patulum plants were collected from a wild source growing at high altitude in the eastern Himalayas. Multiple buds were initiated from shoot-tips cultured on Murashige and Skoog's medium supplemented with BAP, kinetin. Addition of thiamin HCI, Ca-pantothenate and biotin enhanced multiple shoot formation. Upon transfer to phytohormone free liquid medium following a brief exposure to auxin, root formation occurred from the micro shoots . Rooted plants were hardened and transferred to soil. Regeneration potentiality was found to be constant throughout the year in long term cultures.
Subject(s)
Adenine/analogs & derivatives , Biotin/metabolism , Culture Media , Hypericum/physiology , Indoleacetic Acids/pharmacology , Kinetin , Pantothenic Acid/metabolism , Plant Growth Regulators/pharmacology , Plant Roots/growth & development , Plant Shoots/growth & development , Plants, Medicinal/physiology , Purines/pharmacology , Regeneration/physiology , Thiamine/metabolism
2.
Indian J Exp Biol
;
1973 Mar; 11(2): 114-6
Article
in English
| IMSEAR
| ID: sea-58147